In traditional bottom-up experiments, proteins are initially extracted and digested by sequence-specific proteases. The resulting peptides are subsequently analyzed by liquid chromatography (LC) typically coupled to the mass spectrometer through an electrospray interface. The sample preparation prior to LC−MS analysis is a critical part of the overall workflow as it highly impacts sensitivity, robustness, and reproducibility of any bottom-up proteomics approach.

The Preciex® Cleanup tip columns use a proprietary mixed-bed Resin to clean peptide mixtures, specialize in high peptides recovery and efficiently remove detergents, polymers, salts, lipid and more for reliable LC-MS analyses. For detergents such as CHAPS, Igepal, SDC, SDS, Triton X-100 and the polymer PEG-10k the tip removed >99.5% of the contamination; for Tween-20, the removal efficiency was >85%, due to the high repeat numbers of polytheylene glycol.

Process of use: centrifugation, vacuum, positive pressure.

Formats: 10µL, 200µL, and 1mL.

For any other format or online columns, contact us.

*200µL tips are available for different Liquid Handling Automation systems (Zymark, Multiprobe, Tecan, Hamilton, Gilson, Qiagen, and many more) without any further adaptor needed.

Features of Cleanup Tip Columns:

• Dual cleanup step removes hydrophilic and hydrophobic contaminants
• Achieve ready-to-measure peptides
• Reduce mass spectrometry downtime

• Suitable for manual and automated processing

Common product Specifications:

Ordering Information:

User Guide

https://www.freshbioscience.com/public/upload/202212/20221205130519.pdf

Material Safety Data Sheets (MSDS)