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Cleanup Column


In traditional bottom-up experiments, proteins are initially extracted and digested by sequence-specific proteases. The resulting peptides are subsequently analyzed by liquid chromatography (LC) typically coupled to the mass spectrometer through an electrospray interface. The sample preparation prior to LC−MS analysis is a critical part of the overall workflow as it highly impacts sensitivity, robustness, and reproducibility of any bottom-up proteomics approach.

The Preciex® Cleanup tip columns use a proprietary mixed-bed Resin to clean peptide mixtures, specialize in high peptides recovery and efficiently remove detergents, polymers, salts, lipid and more for reliable LC-MS analyses. For detergents such as CHAPS, Igepal, SDC, SDS, Triton X-100 and the polymer PEG-10k the tip removed >99.5% of the contamination; for Tween-20, the removal efficiency was >85%, due to the high repeat numbers of polytheylene glycol.

Process of use: centrifugation, vacuum, positive pressure.

Formats: 10µL, 200µL, and 1mL.

For any other format or online columns, contact us.

 

*200µL tips are available for different Liquid Handling Automation systems (Zymark, Multiprobe, Tecan, Hamilton, Gilson, Qiagen, and many more) without any further adaptor needed.

 

 

Features of Cleanup Tip Columns:

• Dual cleanup step removes hydrophilic and hydrophobic contaminants
• Achieve ready-to-measure peptides
• Reduce mass spectrometry downtime

• Suitable for manual and automated processing

Common product Specifications:

   

Sample

peptide

Chromatographic bed

N/A

Volume

200μL

Capacity

10ug

PH Range

2-12

Temperature

4-70°C

Quantity

96 Tips

Detection Method

HPLC, Mass spectrometry, Capillary electrophoresis, Atomic absorption spectroscopy


Ordering Information:

Description

Format

Capacity

Part Number

Preciex Cleanup Column

10μL

2μg

2107021

Preciex Cleanup Column

200μL

10μg

2107102

Preciex Cleanup Column

200μL

30μg

2107303

Preciex Cleanup Column

200μL

100μg

2107104

  

User Guide

https://www.freshbioscience.com/public/upload/202212/20221205130519.pdf

Material Safety Data Sheets (MSDS)