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Fractionation Column


Recent advances in mass spectrometric instrumentation enable the identification and quantification of peptides and phosphopeptides at an unprecedented depth. Despite the ever increasing sequencing speed of modern mass spectrometers, single measurements are often not sufficient to fully resolve the (phospho)proteome complexity and thus coverage must generally be improved by some form of two-dimensional peptide fractionation.

 

In shotgun proteomics, the most widely applied second dimension of peptide separation is reversed-phase C18 chromatography directly coupled to tandem mass spectrometry. The Preciex® Fractionation columns provide several orthogonal first dimension separations, i.e. SCX, Basic RP, and Hilic, which allow for enhancing specific proteome sample deep coverage by online and/or offline mode.

 

Process of use: centrifugation, vacuum, positive pressure.

Formats: 10µL, 200µL, and 1mL.

For any other format or online columns, contact us.

 

 

*200µL tips are available for different Liquid Handling Automation systems (Zymark, Multiprobe, Tecan, Hamilton, Gilson, Qiagen, and many more) without any further adaptor needed.

 

 

Features of Fractionation Tip Columns:

• Easy-to-use
• Higher recovery of low amount sample
• Various chromatographic mechanisms for the best solution

• Suitable for manual and automated processing

Common product Specifications:

Sample

peptide

peptide

peptide

Chromatographic bed

SCX, 120Å

BRP18, 120Å

HILIC, 120Å

Volume

200μL

200μL

200μL

Capacity

10ug

10ug

10ug

PH Range

2-8

1-12

2-8

Temperature

4-60°C

4-60°C

4-60°C

Quantity

96 Tips

96 Tips

96 Tips

Detection Method

HPLC, Mass spectrometry, Capillary electrophoresis, Atomic absorption spectroscopy


Ordering Information:

Description

Format

Capacity

Part Number

Preciex Fractionation Column, SCX

10μL

2μg

2103021

Preciex Fractionation Column, SCX

200μL

10μg

2103102

Preciex Fractionation Column, SCX

200μL

30μg

2103133

Preciex Fractionation Column, SCX

200μL

100μg

2103104

Preciex Fractionation Column, SCX

1000μL

500μg

2103505

Preciex Fractionation Column, BRP

10μL

2μg

2104021

Preciex Fractionation Column, BRP

200μL

10μg

2104102

Preciex Fractionation Column, BRP

200μL

30μg

2104133

Preciex Fractionation Column, BRP

200μL

100μg

2104104

Preciex Fractionation Column, BRP

1000μL

500μg

2104505

Preciex Fractionation Column, Hilic

10μL

2μg

2105021

Preciex Fractionation Column, Hilic

200μL

10μg

2105102

Preciex Fractionation Column, Hilic

200μL

30μg

2105133

Preciex Fractionation Column, Hilic

200μL

100μg

2105104

Preciex Fractionation Column, Hilic

1000μL

500μg

2105505

 

User Guide

http://freshbioscience.com/public/upload/202207/20220728215335.pdf

Material Safety Data Sheets (MSDS)