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Phosphopeptide Stage-tip


Phosphorylation is among the most widespread post-translational modifications in nature, and it has been estimated that more than 30% of the proteins in a given mammalian cell at some point during their expression are phosphorylated. Phosphorylation and dephosphorylation of proteins regulates a large number of biological processes such as signal transduction, molecular recognition and interaction, and other cellular events. Phosphoproteome mapping can be challenging due to low abundance, dynamic and much less ionization efficiency.

The Preciex® Phosphopeptide enrichment Stage-tip uses a TiO2 Resin with optimized protocol and buffers resulting in a higher yield of phosphopeptides ready for direct MS analysis without the need for additional clean up. It enhances the ability to routinely identify and characterize large numbers of phosphorylated species within complex protein mixtures.

 

Process of use: centrifugation, vacuum, positive pressure.

Formats: 10µL and 200µL.

For any other format or online columns, contact us.

 

 

*200µL tips are available for different Liquid Handling Automation systems (Zymark, Multiprobe, Tecan, Hamilton, Gilson, Qiagen, and many more) without any further adaptor needed.

 

 

Features of Phosphopeptide Stage-tip:

• Higher selectivity for multiply Phosphorylate peptides
• Achieve ready-to-measure Phosphopeptides

• Suitable for manual and automated processing

Common product Specifications:

Sample

Phosphopeptide

Chromatographic bed

TiO2 resin

Volume

200μL

Capacity

4ug

PH Range

1-14

Temperature

4-40°C

Quantity

96 Tips

Detection Method

HPLC, Mass spectrometry, Capillary electrophoresis, Atomic absorption spectroscopy


Ordering Information:

Description

Format

Capacity

Part Number

Preciex Phosphopeptide

10μL

1μg

2108011

Preciex Phosphopeptide

200μL

4μg

2108042

Preciex Phosphopeptide

200μL

15μg

2108153

 

User Guide

http://freshbioscience.com/public/upload/202207/20220728214952.pdf

Material Safety Data Sheets (MSDS)